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Non-Linear Raman Image Contrast Enhancement

Increase signal-to-noise ratio for non-invasive and quantitative chemical microscopy

Description

This microscope imaging technique dramatically enhances the chemical contrast of broadband non-linear Raman images by removing unwanted background signals and retaining resonant signals of interest. The approach uses pulse shaping and closed-form signal analysis to enhance image contrast of broadband non-linear Raman spectroscopy, such as coherent anti-Stokes Raman scattering (CARS) microscopy.  Non-resonant background signals are suppressed by a compact pulse shaper to improve sensitivity and signal-to-noise ratio, thus providing high-contrast and high-sensitivity broadband non-linear Raman microscopy.

Images

microspectrometer design

View of device Credit: NIST

Microspectrometer

Close-up of device Credit: NIST

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Applications

  • Enhanced chemical contrast in medical devices
    Raman spectra are used in disease diagnosis. Nonlinear Raman scattering is more efficient and can lead to the development of diagnostic tools based on Raman spectroscopy imaging

Advantages

  • Improved sensitivity and signal-to-noise ratio
    Suppresses non-resonant background (NRB) in broadband coherent anti-Stokes Raman scattering (CARS) microscopy

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Abstract

The invention disclosed herein is a novel and simple method of suppressing non-resonant background (NRB) in broadband coherent anti-Stokes Raman scattering (CARS) microscopy to improve sensitivity and signal-to-noise ratio.

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Citations

  1. Y. Jong Lee and M.T. Cicerone. Vibrational dephasing time imaging by time-resolved broadband coherent anti-Stokes Raman scattering microscopy. Appl. Phys. Lett. 92, 041108 (2008). doi:10.1063/1.2838750
  2. Y.J. Lee and M.T. Cicerone. Single-shot interferometric approach to background free broadband coherent anti- Stokes Raman scattering spectroscopy. Optics Express, Vol. 17, Issue 1, pp. 123-135 (2009). doi:10.1364/OE.17.000123
  3. Y.J. Lee and M.T. Cicerone. Pulse shaping for background free broadband CARS. Proceedings of SPIE, Vol. 7183, pp. 71830Y-71830Y-8, 2009. doi: 10.1117/12.809102
  4. Y.J. Lee, Y. Liu, and M.T. Cicerone. Characterization of three-color CARS in a two-pulse broadband CARS spectrum. OPTICS LETTERS, Vol. 32, No. 22, November 15, 2007.

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References

Status of Availability

active patent and available for licensing

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