NIST Technicalendar March 5 to March 9, 2007 The NIST Technicalendar is issued each Friday. All items MUST be submitted electronically from this web page by 12:00 NOON each Wednesday unless otherwise stated in the NIST Technicalendar. The address for online weekly editions of the NIST Technicalendar and NIST Administrative Calendar is: http://www.nist.gov/tcal/.
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In this Issue: Meetings at NIST Meetings Elsewhere Announcements Talks by NIST Personnel NIST Web Site Announcements NIST Administrative Calendar (current)   NIST Vacancy Announcements (current)

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No Scheduled Events

No Scheduled Events

1:30 PM - Carbohydrate Microarrays for Basic and Translational Cancer Research

10:30 AM - Nanomedicine: Nanotechnology for Enhancing Human Health
10:45 AM - Predicting the In-Service Performance of Elastomers, A Surprisingly Complicated Problem
1:00 PM - Inhibiting enzymes by mechanical deformation: single molecule fluorescence studies of serpins
2:30 PM - In Vivo Microscopic Imaging of the Kidney for New Functional Diagnostics

No Scheduled Events

3/5 -- MONDAY

No Scheduled Events

3/6 -- TUESDAY

No Scheduled Events

3/7 -- WEDNESDAY

1:30 PM - PROCESS MEASUREMENTS DIVISION SEMINAR: Carbohydrate Microarrays for Basic and Translational Cancer Research
J. Gildersleeve , National Cancer Institute.
221 Bldg, Rm. A366. (NIST Contact: M. Tarlov, 301-975-2058, mtarlov@nist.gov)


3/8 -- THURSDAY

10:30 AM - NIST COLLOQUIUM SERIES : Nanomedicine: Nanotechnology for Enhancing Human Health
NOTE: ****DIFFERENT DATE***** The application of nanotechnology to the prevention and treatment of human diseases holds great promise, but nanomaterials must be biocompatible, non-toxic and functional in biologic (wet) conditions, and well enough defined for acceptance by regulatory agencies. Early applications of nanomaterials will likely involve the development of medications that take advantage of unique aspects of nanostructures to achieve or enhance therapeutic activity. Examples will be given for the design, synthesis, and analysis of nanomaterials having attached molecules that allow for unique therapeutic functions. Applications include antimicrobial compounds, drug and gene delivery, and functional imaging. Concepts for future applications such as cellular engineering, human performance augmentation, and genetic manipulation for the treatment of human disease will be presented.
James Baker, Jr., M.D. , Director, Nanotechnology Institute for Medicine and the Biological Sciences, University of Michigan.
Administration Building, Red Auditorium. (NIST Contact: Kum Ham, 301-975-4203, kham@nist.gov)
Special Assistance Available

10:45 AM - MATERIALS AND CONSTRUCTION RESEARCH DIVISION SEMINAR SERIES: Predicting the In-Service Performance of Elastomers, A Surprisingly Complicated Problem
It starts with a simple question. What is the best elastomeric material for an application exposed to the weather? The answer, like the behavior of the material, is complex involving the integration of rheology, reliability based methodology, analytical chemistry, mechanical engineering, statistics, standards development, and economics. Starting from this simple question the current methods, their consequences, development and implantation of new methods are examined. In this research the affect of controlled temperature, humidity, Ultra-Violet radiation, and applied strain is used to “age” model elastomers in a custom SPHERE device (simulated photo degradation due to high energy radiant exposure, a device that generates >25 “suns” of Ultra-Violet radiation). Additional verification is provided by long term outdoor exposure. The chemical changes related to aging are monitored through changes in the complex modulus. Consideration of the Mullins affect and movement during curing will be examined. It will also be shown that applying strain to an elastomer affects the solubility. This surprising result can either increase or decrease the solubility, contrary to Treloar’s finding of natural rubber in good solvents. Additionally, the viscous dissipation within these elastomers makes determination of the actual strain level an important consideration. It now seems possible to predict the in-service performance of elastomers and polymeric coatings based on laboratory experiments.
Christopher White , Polymeric Materials Group.
224 Bldg, Rm. B245. (NIST Contact: Aaron Forster, 301-975-8701, aaron.forster@nist.gov)
Special Assistance Available

1:00 PM - OPTICAL TECHNOLOGY DIVISION SEMINAR: Inhibiting enzymes by mechanical deformation: single molecule fluorescence studies of serpins
Inhibitory serpins regulate serine and cysteine proteases involved in blood coagulation, fibrinolysis, inflammation and other important physiological processes. In the unique serpin inhibitory mechanism, the target protease docks with the serpin's exposed reactive center loop (RCL). Upon cleavage, the RCL inserts into an existing serpin ? sheet towing the protease, which is covalently attached to the cleaved RCL, 70 Å from one pole of the serpin to the other. The force exerted by the structural remodeling of the serpin pulls the catalytic serine 3 Å out of position inhibiting the protease. All of the energy required for the extensive remodeling of both the serpin and protease structure is stored in the initial, metastable serpin structure. Using single molecule fluorescence resonance energy transfer, we have investigated the conformational distributions of trypsin-??-antitrypsin complexes as well as ??-antitrypsin folding. Our results show that intermediates in complex formation can be trapped, and that trypsin is partially translocated as well as partially disrupted in these intermediates. Our unfolding studies, aimed at elucidating how serpins fold to the metastable structure while bypassing more energetically favorable structures, suggest that the folding intermediate is more spherical than the ellipsoidal metastable ??-antitrypsin conformation. Such symmetric folding intermediates may be favored by asymmetric proteins. The trypsin-??-antitrypsin studies show that full translocation is not required for the formation of metastable protease-serpin complexes while the folding studies provide a glimpse of the intermediate and unfolded conformational distributions.
Ann Gershenson , Assistant Professor, Dept. of Chemistry, Brandeis University, Waltham, MA, gershenson@brandeis.edu.
AML Bldg, Room H107. (NIST Contact: Lori Goldner, 301-975-3792, lori.goldner@nist.gov)

2:30 PM - OPTICAL TECHNOLOGY DIVISION SEMINAR: In Vivo Microscopic Imaging of the Kidney for New Functional Diagnostics
Normal functions of the kidney are directly associated with filtration, reabsorption and excretion for the regulation of salt and water content of the body, removing waste products and maintaining chemical balance of the blood. Intravital two-photon fluorescence microscopy holds particular promises to study these processes as it allows observations of local regions of the kidney, such as the glomerulus, proximal tubule and distal tubule cells with subcellular and submicron resolutions and at the same time with nanomolar concentration sensitivity. The parameters we are able to evaluate with intravital microscopy are comprehensive. Here I will discuss some of the quantitative approaches and techniques including the use of quantum dots nanoprobes we apply to study kidney functions and illustrate that the knowledge we acquired with in vivo microscopic imaging will have direct and major impact on future drug design and the development of new diagnostic methods.
Dr. Weiming Yu , Assistant Professor of Medicine, Indiana University,, Indianapolis, IN, wmyu@iupui.edu.
AML Bldg, Rm. H107. (NIST Contact: Dr. Jeeseong Hwang, 301-975-4580, jch@nist.gov)


3/9 -- FRIDAY

No Scheduled Events

ADVANCE NOTICE

3/13/07 2:00 PM - ELECTRON AND OPTICAL PHYSICS DIVISION SEMINAR: Matrices of single-photon avalanche diodes for biomolecular analysis
In this talk I will describe my work at Politecnico di Milano related to the design of analysis systems for DNA and proteins, based on single photon avalanche photodiodes (SPADs). These devices are easy to integrate and miniaturize, so are suitable for for low cost portable biological equipment. I first report a demonstration of the use of SPADs in microchip electrophoresis with single and multi-wavelength laser induced fluorescence. The development of monolithic matrices or arrays of SPADs enables parallel single photon counting on different channels leading to higher throughput and the possibility of multi-wavelength photon counting. Looking at these possibilities I designed a 6x8 matrix of SPADs intended for parallel chemiluminescence and I built a complete and compact optical and mechanical system for automated analisys of protein microarrays. The integration of SPADs on the same silicon chip leads to issues regarding the electrical and optical crosstalk between the devices. I discuss how it is possible to completely eliminate the first effect, and I show measurements of the optical crosstalk using coincidence counting techniques. Today, state of the art equipment for DNA and protein analysis is based on charge-coupled device cameras or confocal scanning microscopes with photomultipliertubes. Both such systems work in analogue mode, i.e. the detector output is an electrical quantity proportional to the photon emission rate. This provides sensitivity down to single molecule levels, but single-photon counting techniques can often provide better sensitivity and provide for advanced measurement techniques such as sinusoidal lock-in in the shot-noise limited regime, and fluorescence correlation Spectroscopy. As a demonstration of advanced single photon counting techniques I designed and tested a novel full-digital sinusoidal lock-in amplifier designed to operate with single photon events. Experimental results show that it is essentially limited by shot noise and can work with extremely low photon count rates.
Alessandro Restelli , Research Associate, Electron and Optical Physics Division.
Radiation Physics Bldg, Rm. B105. (NIST Contact: Charles Clark, 301-975-3709, charles.clark@nist.gov)
Special Assistance Available

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MEETINGS ELSEWHERE


3/5 -- MONDAY

No Scheduled Events

3/6 -- TUESDAY

4:15 PM - THE JOHNS HOPKINS UNIV. CHEMISTRY DEPT/ EPHRAIM AND WILMA SHAW ROSEMAN COLLOQUIUM SERIES: A POST-PROTEOMIC APPROACH TO STUDY RIBOSOME ASSEMBLY
J. Williamson , Scripps.
Bldg, Rm. .
Chemistry Dept., The Johns Hopkins Univ., Baltimore, MD. (NIST Contact: R. Elder, 410-516-7432, rosalie@jhu.edu)



3/7 -- WEDNESDAY

No Scheduled Events

3/8 -- THURSDAY

No Scheduled Events

3/9 -- FRIDAY

No Scheduled Events

ADVANCE NOTICE

4/24/07 9:00 AM - COMPACT X-RAY SOURCES BASED ON INVERSE COMPTON-SCATTERING OF LASER RADIATION
Satellite Workshop to the 14th National Synchrotron Radiation Instrumentation Conference SRI2007
Winthrop Brown , MIT Lincoln Laboratory, Cambridge, MA, wbrown@ll.mit.edu. Frank Carroll , MXISystems, Inc., Fairview, TN, frank.carroll@mxisystems.com. Ronald Ruth, Lyncean Technologies, Inc., Palo Alto, CA, ronald_ruth@lynceantech.com and David Moncton, MIT Nuclear Reactor Laboratory, Cambridge, MA, dem@mit.edu
Bldg, Rm. .
Hilton Baton Rouge Capitol Center, Baton Rouge, Louisiana. (NIST Contact: Uwe Arp, 301-975-3233, uwe.arp@nist.gov) http://www.camd.lsu.edu/SRI/workshops.htm

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TALKS BY NIST PERSONNEL

BEVERSLUIS, M. : NANO OPTICS FOR CHEMICAL AND MATERIALS CHARACTERIZATION.
American Physical Society Meeting, Colorado Convention Center, Denver, Co, 3/5.

LI, Q. (Co-Author: C.Richter ) : NANOWIRE NON-VOLATILE MEMORY WITH SILICON NITRIDE CHARGE TRAPPING LAYER.
2007 APS March Meeting, Denver, CO, 3/5.

WANG, W. (Co-Author: C.Richter ) : SEMICONDUCTOR ELECTRONICS.
American Physical Society March Meeting, Denver, CO, 3/5.

ROBEY, S. : EFFECTS OF -NO2 SUBSTITUTION ON CHARGE ADDITION AND REORGANIZATION ENERGIES IN PHENYLENE ETHYNYLENE OLIGOMERS.
American Physical Society Meeting, Colorado Convention Center, Denver, CO, 3/5.

RICHTER, L. : IN-SITU CHARACTERIZATION OF THE MESOPHASE OF HIGH PERFORMANCE SEMICONDUCTING POLYMER.
American Physical Society Meeting, Colorado Convention Center, Denver, CO, 3/5.

SCHOENMAKER, J. : STRESS IMAGING IN INDENTED SI WAFERS BY CONFOCAL RAMAN MICROSCOPY .
American Physical Society Meeting, Colorado Convention Center, Denver, CO, 3/6.

SHIRLEY, E. : GW/BETHE-SALPETER CALCULATIONS FOR SOLIDS FOR CORE AND VALENCE SPECTRA AMERICAL PHYSICAL SOCIETY, DENVER, CO..
Eric Shirley, Denver, CO, 3/6.

GADZUK, J. : ON THE ROLE OF MULTIPLE HOT ELECTRON PROCESSES IN STM-INDUCED ATOM MOTION ON SURFACES.
American Physical Society, Denver, CO, 3/6.

SIMPSON, J. : TEMPERATURE-DEPENDENT RAMAN SPECTROSCOPY OF MULTIFERROIC TBMN2O5.
American Physical Society March Meeting, Colorado Convention Center, Denver, CO, 3/7.

RICHTER, C. (Co-Authors: C.Hacker N.Gergel-Hackett ) LJ Richter, TC Allison, V Mujica (Northwestern), and CA Gonzalez : CHARACTERIZATION OF ELECTRICAL DEVICES BASED UPON ORGANIC MONOLAYERS DIRECTLY ATTACHED TO SI.
2007 March Meeting of the American Physical Society, Denver, Colorado, 3/8.

SHIRLEY, E. : ASSIGNING SPECTRAL FEATURES IN EXCITONIC SPECTRA.
American Physical Society, Denver, Co, 3/8.

HIGHT WALKER, A. : RESONANCE RAMAN SPECTROSCOPY OF LENGTH FRACTIONATED SINGLE-WALLED CARBON NANOTUBES.
American Physical Society, Colorado Convention Center, Denver, CO, 3/8.

YU, L. : CHARGE MODIFICATION OF VIBRATIONAL FEATURES IN INELASTIC ELECTRON TUNNELING SPECTROSCOPY.
American Physical Society Meeting, Colorado Convention Center, Denver, CO, 3/9.

KUSHMERICK, J. : ELECTRONIC AND VIBRONIC SPECTROSCOPY OF MOLECULAR JUNCTIONS.
American Physical Society Meeting, Colorado Convention Center, Denver, CO, 3/9.

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ANNOUNCEMENTS

NVLAP ASSESSOR SEMINAR
The National Voluntary Laboratory Accreditation Program (NVLAP) accredits testing and calibration laboratories that are found competent to perform specific tests and calibrations. NIST Handbook 150, Procedures and General Requirements, and ISO/IEC 17025, General Requirements for the competence of testing and calibration laboratories, will be presented to current NVLAP assessors, new assessors and others that are interested in laboratory accreditation. Seminar size is limited to 20. Anyone interested in attending should contact Betty Ann Sandoval as soon as possible.
NIST Contact: Betty Sandoval, 301-975-8446, betty.sandoval@nist.gov

VISITOR REGISTRATION FOR NIST EVENTS
Because of heightened security at the NIST Gaithersburg site, members of the public who wish to attend meetings, seminars, lectures, etc. must first register in advance. For more information please call or e-mail the "NIST Contact" for the particular event you would like to attend.
NIST Contact: . ., ., .

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NIST WEB SITE ANNOUNCEMENTS

No Web Site announcements this week.

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For more information, contact Ms. Sharon Hallman, Editor, Stop 2500, National Institute of Standards and Technology, Gaithersburg MD 20899-2500; Telephone: 301-975-TCAL (3570); Fax: 301-926-4431; or Email: tcal@nist.gov.

All lectures and meetings are open unless otherwise stated.

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